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KMID : 0545120100200071101
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1101 ~ p.1106
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Accumulation of Selenium and Changes in the Activity of Inulinase and Catalase in the Cells of Kluyveromyces marxianus on Pulsed Electric Field Treatment
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Urszula Pankiewicz
Jerzy Jamroz
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Abstract
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Pulsed electric field (PEF) of 1Hz, 1.5 kV, and 1ms increased the activities of catalase and inulinase over the whole range of applied Se concentrations compared with the non-treated cultures. A significant effect of selenium concentration (in the range of 5-14 μg/ml) on both intraand extracellular enzyme activities was noted. At a Se concentration of 10 μg/ml, the activities of intra- and extracellular inulinases and extracellular catalase in the PEF-treated cultures reached the maximum of 71 U/g d.m., 46 U/g d.m., and approx. 8 U/ml, respectively. The maximum activity of intracellular catalase of approx. 6 U/ ml (with and without PEF) was recorded at 5 μg Se/ml. Further increasing of selenium concentration caused a decrease in the activity of the enzymes.
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KEYWORD
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selenium, Kluyveromyces marxianus, inulinase, pulsed electric field
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